'From cat 1. D.N., Deiters' nucleus. S.O., superior olive. I.P.C., inferior peduncle of cerebellum.' (192)


Fig. in text:

'The changes which I have noticed are as follows:- In most of the affected cells there is a total absence of Nissl's granules (compare Fig. 3, A and B), in many there is a diminution in the number of these granules while in a few cells there is little or no alteration. The difference is best seen when the cells are deeply stained and the sections examined under a very low power of the microscope; under these circumstances the appearances shown in Figs. 1 and 2 are seen. If the comparison between the two sides is made from the alteration in the appearance of the cells rather than by counting the number of cells visible, one principal source of error is overcome, namely that arising from the difficulty of obtaining strictly transverse sections; for the cells diminish in number towards the upper (or anterior), and lower (or posterior) part of the nucleus, and if the sections are not completely transverse, error is likely to occur from comparing the middle of one nucleus with the upper or lower part of the other, especially as the extent of the nucleus from above down is rather limited (a series of about 50 thick sections will include the whole nucleus). But if the appearance of the cells is compared this error does not occur, for in comparing the middle of the degenerated nucleus with the upper or lower part of the undegenerated, the difference can be clearly seen even although the cells appear more numerous on the side which has undergone degeneration.' (192-194)